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METABOLISM UNIT

   
  MICHAEL JURCZAK, PHD
Director of
Rodent Phenotyping
 
     
     

PLATFORM TECHNOLOGIES

Mouse Metabolic Monitoring

The Comprehensive Lab Animal Monitor System (CLAMS) allows for the continuous and simultaneous monitoring of indirect calorimetry (VO2, respiratory exchange ratio), food intake, and physical activity in mice. The CLAMS is configured for eight individually housed test animals that comply with the USDHHS guidelines for long-term housing.  Data are exported in .csv format for use in multiple spread-sheet applications.

Mouse Body Composition

An EchoMRI body composition analysis system allows for measures of whole body fat, lean, free water, and total water mass in living, conscious animals. Results can be obtained from organs or mice (up to 130 g) in as little as 30 seconds.

Exercise Testing with CLAMS

Acute exercise testing in mice can be performed using a single-lane air-tight enclosed treadmill used in conjunction with the CLAMS for respiratory metabolic assessment during exercise. Integrated software allows for user-defined protocol interval and speed determination. The unit also features incline capability in 5° increments.

Exercise Testing in Open Air

Acute or chronic (training) exercise protocols can be performed in an open-air treadmill featuring three (rats) or six (mice) lanes, and includes adjustable incline capabilities (0-25°). User-friendly software allows for user-defined protocols, adjustable acceleration (in 0.1 meter/minute-steps per second), and wide range of speed (3-100 meters/minute). Data are exported in .csv format.

Bomb Calorimetry

The use of bomb calorimetry allows for the assessment of energy content in both food and/or feces to determine the energy intake of study animals. The Parr 6200 is an isoperibol oxygen bomb calorimeter that can analyze 8-9 samples per hour.

Mitochondrial Respiration

An OROBOROS O2k, fitted with two experimental chambers, is used for high-resolution respirometry and the quantitative assessment of oxygen flux in biological samples.. Multiple substrates-uncoupler-inhibitor titration (SUIT) protocols can be applied for the study of cell respiration or mitochondrial activity. The unit is also fitted with a fluorometer for the assessment of reactive oxygen species production. Typical protocols can be completed in 2-4 hours.

Insulin Sensitivity by Hyperinsulinemic Euglycemic Clamp

The hyperinsulinemic euglycemic clamp is the gold-standard for determining insulin sensitivity in vivo. By using metabolic isotopic tracers during clamp, whole-body and tissue-specific rates of carbohydrate and lipid metabolism can be measured under fasted and insulin-stimulated conditions in transgenic mice. Key parameters measured: basal rates of hepatic glucose production and whole-body glucose utilization; insulin-stimulated rates of hepatic glucose production and whole-body glucose uptake; tissue-specific rates of glucose uptake; fasting and insulin-stimulated plasma fatty-acid levels and palmitate turnover as indices of adipose tissue insulin sensitivity.


CONTACT

Michael Jurczak, PhD | jurczakm@pitt.edu
Rob O'Doherty, PhD | rmo1@pitt.edu



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